|
KMID : 0380020070220020114
|
|
Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 2 p.114 ~ p.118
|
|
|
Isolation and Characteristics of an Amylase-producing Fungus for Saccharifying Food Wastes
|
|
Li Hong-Xian
Kim Seong-Jun
|
|
|
Abstract
|
|
|
|
In this study, an amylase-producing fungus, strain 15 was isolated from soil in order to saccharify food wastes with cellulolytic and amylolytic enzymes. The amylase production cultures were performed in Mandel`s medium with 1% rice straw and 1% paper wastes as carbon sources. The strain produced various cellulolytic (FPase 0.25, xylanase 20.09, CMCase 3.15 U/mL-supernatant) and amylolytic (α-amylase 1.20, gluco-amylase 0.70, β-amylase 2.40 U/mL-supernatant) enzymes in Mandel`s medium. In 10 L jar fermenter, maximum amylase and FPase activities, 3.25 and 0.23 U/mL, were obtained when the culture was grown at 30℃, 200 rpm and 0.6 vvm for 3 days. In 100 mL flask level and 10 L jar fermenter, amylase produced by the strain 15 showed similar cellulolytic and amylolytic enzyme activities with Trichoderma inhamatum KSJ1 isolated from rotten woods by previous researcher. The ability of saccharification to food wastes also showed similar degree. However, the isolate 15 appeared to be yellowish in YMEA plate comparing to Trichoderma inhamatum KSJ1 in greenish.
|
|
|
KEYWORD
|
|
|
Amylase, fungus, saccharification, food wastes
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|